In vitro enzymatic synthesis of new penicillins containing keto acids as side chains.
نویسندگان
چکیده
Seven different penicillins containing alpha-ketobutyric, beta-ketobutyric, gamma-ketovaleric, alpha-ketohexanoic, delta-ketohexanoic, epsilon-ketoheptanoic, and alpha-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum with CoA, ATP, Mg(2+), dithiothreitol, 6-aminopenicillanic acid, and the corresponding side chain precursor.
منابع مشابه
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 35 9 شماره
صفحات -
تاریخ انتشار 1991